Sunday, June 23, 2013

La Semana Andre: A Delayed Posting

I apologize for the delay in getting this post up! I actually had it written last week when I should have posted it, but this last week has been an insane amount of work. You'll hear all about it in my next blog post, which is not yet written but I hope to have up today or tomorrow. Now with that disclaimer, on to the rest of the story!

Hola mis amigos!

It's been another very busy week here in Panama! The first, and most exciting thing, is that we have the water turned on! After two weeks of building, this felt like a massive accomplishment. But let me start at the beginning.
 
Last Sunday one of Amber's friends, named Greg, arrived to help us for a week. He's a teacher at South Hampton High School, and he's in charge of the marine biology electives and the lab they have. He came down for a week to help us build gutters on the tables, figure out water flow, and organize all of the electronic measurement systems.
 
The gutters are necessary because each tank, to maintain water flow, has a tube going in and then just overflows onto the table. It's really not as much fun to do all the measurements and such if your feet get wet every time, and the salt water could easily corrode the metal table legs by December (which is the end date of the project). So to fix this problem, we tilted the tables slightly and screwed a PVC pipe with a slit cut out of it to each table. Now there are only minimal drips! This is the only part of the project we've had to re-do; the first time we cut the PVC pipes in half, and there was simply too much water and they were overflowing. The second time we cut about an inch- wide strip out of the circumference, and that worked much better.
 
I also spent a lot of this week labeling things. Each rain barrel and each tank got a color-coded label that was numbered, and we also wrote the tank numbers on the table so when we moved the tanks it would be easy to put them back. We also cut tubing of appropriate length to feed the rain barrels from the wet lab water system, and tubing to go from the rain barrels to the individual tank. Each one of the eighteen rain barrels feeds twelve tanks. All of this tubing also needed labeling; can you imagine the mess we would have if we got some of the tubing, cut to length, mixed up without a label on it?
Once we had all of this done (with the first round gutters), we got to turn on the water for one of the tables. This was when we realized the flaw in our gutter design, and we also realized that the tanks couldn't just overflow over the top; they were flowing into each other and would contaminate the different treatments. To rectify this problem, Sarah and Greg spent an afternoon drilling half-inch holes in the tanks. It's incredible how long stuff takes when you have to do it 216 times!
 
Once we had the drilled tanks back in place, the tubing labeled and put in the right tank, we turned the water on for the whole system. It was a really fantastic thing to watch everything fill up! But then, of course, came the adjustments. This is going to be my life for the rest of my time here, I think.
 
Because the whole water system for the wet lab is based on tides, you get varying water flow depending on whether or not it's high tide or low tide. You also get sediment and shells and things flowing through the system, which can clog the valves. For Amber's experiment, we need about 0.5 liter per minute of water flow in each tank, which means we're pretty much maxing out the water we can get. Also, because the flow from the rain barrels to the tanks is driven by gravity, we need to have enough water in the barrels to maintain pressure so the water keeps flowing. When there isn't enough of this pressure (called head pressure), the tubes can get air-locked, which is when the water doesn't have enough pressure to push the air in the tube out. Each tube, also, has a slightly different flow rate due to how long it is, what side of the barrel it's on, and basically just the tricky nature of the thing.
 
Greg and I spent nearly two days adjusting each of the 216 valves, and we definitely don't have them all dialed in yet. Maintaining head pressure in the barrels is quite a balancing act; you have to give the animals enough water to live but you can't use more water than is coming in. We're also going to have to clean out the eighteen big valves at least every day to make sure we're getting enough water. But despite all of this fiddliness, I'm still excited. At least we have a system to fiddle with!
 
The other accomplishments for this week include getting all the CO2 tanks secured and tubed and wired with the pH monitors, and setting up all the outlets and extension cords for the pH monitors and the heaters. As soon as we got it all set up, of course, we had to waterproof the whole thing as best we could, which in our case meant using zip ties to secure zip lock bags over everything. We'll see how everything holds up as we go.
 
On Monday, another scientist named Melissa is coming who will be using our system to do molecular work with sponges. She'll be bringing a million and three microscope slides, which will mean the beginning of our last big project; preparing the corals. Each coral needs to get weighed, tagged, and then glued to a microscope slide so it stands upright in the tank. However, each tank is going to get seven corals. 216 times 7 is 1512 pieces of coral, so we'll be busy for a while. Fortunately the sponges don't need any preparation before we put them in the tanks!
 
And lest you all think I'm a total biology dork and all I do down here is talk about marine ecology, I'll tell you a little about our afternoons and evenings. Normally we knock off work around three, before the chichras get too bad. When we get home, we all kind of lounge and read and swim and shower. Because Greg was here this week, we took the opportunity to go out to eat several times. Amber's favorite restaurant is called Ultimo Refugio, and it's absolutely fantastic. We also took Greg back to our sushi place from last week, and one night we simply went out for brownies at a boat-turned-bar called Riptide. After dinner we'd hang about again, watching the sting rays swim around (there's a group of about seven that are here every night) and enjoying the breeze. The nights here are absolutely glorious.
 
This weekend thus far has been pretty mellow; it's raining today so I took the opportunity to do laundry, clean up and organize all my stuff, and catch up on writing. My next project is uploading pictures, so hopefully the post after this will explain the experimental set-up much better than I've been able to in words!
 
Until next time,

Hasta pronto!

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